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Background:
Research
increasingly
suggests that asthma
is a familial and
hereditary disorder
in the pathogenesis
of which genetic and
environmental
factors play an
important role.
Objective: To
investigate the
single and combined
associations between
8 single-nucleotide
polymorphism (SNP)
loci in 5 genes and
the development of
asthma in children
of Chinese Han
nationality.
Methods: The
study population
comprised 192
children with asthma
and an equal number
of healthy controls.
Asthma was diagnosed
in accordance with
American Thoracic
Society criteria.
Polymerase chain
reaction-restriction
fragment length
polymorphism was
used todetect the
genotypes of the SNP
loci.
Results: No
statistically
significant
differences (P>.05)
were found between
the experimental and
control group in
genotype
distribution among 6
loci (IL-13 C−1112T,
IL-13 C1923T, IL-4
C−590T, IL-4RA I75V,
FcεR1ß E237G, and
ß2-ADR Q27E).
However, significant
diversity was
observed among
FcεR1ß C−109T
(P=.002) and ß2-ADR
R16G (P=.000).
Furthermore, the
frequency of FcεR1ß
C−109T T/T and ß2-
ADR R16G A/A in the
asthma group was
significantly higher
than in the control
group (odds ratio
[OR]=1.96, P=.001;
OR=2.58, P=.000,
respectively).
Carriers of both
FcεR1ß C−109T T/T
and ß2-ADR R16G A/A
had a more
significant risk of
developing asthma
than those
with only a single
polymorphism.
Conclusion:
The 6 loci (IL-13
C1112T, IL-13
C1923T, IL-4 C590T,
IL-4RA I75V, FcεR1ß
E237G and ß2-ADR
Q27E) make little
contribution to the
development of
asthma in children
of Chinese Han
nationality. FcεR1ß
C109T and ß2-ADR
R16G are
significantly
associated with
childhood asthma.
FcεR1ß C109T T/T
and ß2-ADR R16G A/A
have a significant
and combined effect
on the development
of asthma.
Key words:
Asthma. Single
nucleotide
polymorphism. IL-13.
IL-4. IL-4 receptor.
Fc receptor.
Adrenoreceptor.
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