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M Ferrer,1 ML Sanz,1
J Sastre,2,3 J Bartra,4,3 A del
Cuvillo,5 J Montoro,6 I Jáuregui,7
I Dávila,8 J Mullol,9,3 A Valero4,3 |
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1 Departamento de Alergia e
Inmunología Clínica, Clínica Universidad de Navarra,
Pamplona, Spain
2 Servicio de Alergia, Fundación Jiménez Díaz, Madrid,
Spain
3 Centro de Investigación Biomédica en Red de
Enfermedades Respiratorias (CIBERES)
4 Unitat dAl.lèrgia, Servei de Pneumologia i Al.lèrgia
Respiratòria, Hospital Clinic (ICT), Barcelona, Spain
5 Clínica Dr. Lobatón, Cádiz, Spain
6 Unidad de Alergia, Hospital La Plana, Vila-Real (Castellón),
Spain
7 Servicio de Alergología, Hospital de Basurto, Bilbao,
Spain
8 Servicio de Inmunoalergia, Hospital Universitario,
Salamanca, Spain
9 Unitat de Rinologia & Clínica de lOlfacte, Servei
dOto-rino-laringologia, Hospital Clínic Immunoal.lèrgia
Respiratòria Clínica i Experimental, IDIBAPS. Barcelona,
Spain |
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Abstract |
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Recombinant and
purified allergens
are currently
available for
determining specific
IgE targeted to
different allergenic
components. In this
way it is possible
to diagnose the
sensitization
profile of each
individual patient.
The microarray
technique makes it
possible to
determine specific
IgE against multiple
allergens
simultaneously in
one same patient,
with a minimum
amount of serum, and
even allows the
determination of IgG
and IgM against the
same allergens in
one same serum
sample. Microarray
procedures are being
developed not only
for the
determination of
antibodies but also
for cell activation
tests. In addition,
microarray
technology will help
explain
cross-reactions, and
will facilitate the
evaluation of
subjects in which
skin tests cannot be
performed. These
techniques will
allow a great step
forward in the
development of
immunotherapy
specifically
targeted to the
sensitizations found
in each individual
patient, yielding
especially
hypoallergenic forms
of great immunogenic
capacity, and thus
improving the safety
and efficacy of
immunotherapy.
Lastly, microarrays
will improve our
understanding of the
physiopathology of
allergic diseases.
Key words:
Microarrays,
diagnosis by
components, specific IgE,
immunotherapy.
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