|
MD Bazzi,1 MA Sultan,2
N Al Tassan,2 M Alanazi,2 A
Al-Amri,2 MS Al-Hajjaj,3 S
Al-muhsen,4 K Alba-Concepcion4, A
Warsy2 |
|
1The Center of Excellence in
Biotechnology Research, King Saud University, Riyadh,
Saudi Arabia
2The Genome Research Chair, Department of Biochemistry,
College of Science, King Saud University, Riyadh, Saudi
Arabia
3The College of Medicine, King Khalid Hospital, King
Saud University, Riyadh, Saudi Arabia
4The Asthma Research chair, Department of Pediatrics,
College of Medicine, King Saud University, Riyadh, Saudi
Arabia |
|
Abstract |
|
Background:
Asthma is a
multifactorial
disorder, and both
genetic and
environmental
factors contribute
to its development.
We investigated the
possible association
between asthma and 5
single-nucleotide
polymorphisms (SNPs)
in the interleukin
17 (IL17)
geners17880588
(G/A) and rs17878530
(C/T) in IL17A and
rs763780 (T/C),
rs11465553 (T/C),
and rs2397084 (G/A)
in IL17Fand
compared levels of
the proteins IL17A
and IL17F in asthma
patients with those
of controls.
Patients and
Methods: The
study group included
100 asthma patients
and 102 ethnically
matched controls.
Genotyping was
performed on
purified DNA using
reverse
transcriptase-polymerase
chain reaction with
specific primers and
probes. Levels of
IL17A and IL17F
weremeasured in
plasma using
enzyme-linked
immunosorbent assay.
Results:
Genotyping showed
that AG
heterozygotes of
rs17880588 in IL17A
were significantly
more common in the
control group than
among the asthma
patients (P<.05); no
significant
associations were
observed for any of
the other SNPs
examined. Levels of
IL17A and IL17F were
both higher in
asthma patients
(IL17A, 2.242
[0.099] vs 2.752
[0.287] pg/mL;
IL17F, 236.01
[38.28] vs 700
[201.078] pg/mL).
The difference was
statistically
signifi cant for
IL17F (P=.025, t
test). Levels of
IL17A and IL17F were
positively and
significantly
correlated in the
asthma patients
Conclusion:
Of all the SNPs
analyzed, only
rs17880588 showed a
significant
association with
asthma in the Saudi
population we
studied. Levels of
IL17A and IL17F were
significantly
upregulated in the
asthma patients. The
morphology of IL17F
appeared to affect
expression levels.
Key words:
Asthma. IL17. Saudi
Arabia. Genotyping.
Gene expression.
RT-PCR.
|
|
|
|
| |
|
|
