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Background:
Immunoglobulin (Ig)
E plays an important
role in the
pathogenesis of
allergic diseases
such as atopic
dermatitis and
allergic asthma. We
previously reported
that a sulfate
polysaccharide,
fucoidan, suppressed
IgE production by
murine B cells in
vitro. However, the
mechanism by which
fucoidan suppresses
IgE production
remains unclear.
Objective: We
incorporated sulfate
groups into
cellulose and
studied their
biological
characteristics in
vitro to explore the
possibility of
converting
biologically neutral
polysaccharides to
active reagents with
antiallergic
functions.
Material and
Methods:
Cellulose was
chemically processed
using N,N
dimethylformamide (DMF)
and
DMF-sulfurtrioxide
and recovered as
cellulose sulfate
with a molecular
weight of around 10
kDa. We then studied
the effect of
cellulose sulfate on
IgE production from
B cells, IgE
class-switching, and
populations of IgE-secreting
B cells prepared
from murine spleen.
We also investigated
the effects of
sulfated cellulose
on the production of
interleukin (IL) 4
and interferon (IFN)
γ and the expression
of T-bet mRNA by
splenic T cells. The
cytotoxicity of
cellulose sulfate
was also examined.
Results:
Cellulose sulfate
suppressed IgE
production in B
cells stimulated
with IL-4 and
anti-CD40 antibody
by inhibiting IgE
class-switch
recombination and
decreasing the
number of IgE-secreting
B cells in vitro.
Moreover, both
cellulose sulfate
and fucoidan
suppressed IL-4
production and
enhanced IFN-γ
production by murine
T cells stimulated
with anti-CD3 and
anti-CD28
antibodies, despite
the decrease in
T-bet mRNA
expression.
Conclusions:
Cellulose gains an
antiallergic effect
on B cells and T
cells with the
addition of sulfate
groups.
Key words:
Cellulose sulfation.
T cells. B cells.
IgE. Class-switch
recombination.
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