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Ahlgrim C1, Gutermuth J2,
Önell A3, Borres MP3,4, Schäffner
I5, Darsow U6,7, Pfab F6,7,
Brockow K6,7, Ring J6, Behrendt H7,
Jakob T8, Huss-Marp J5,8 |
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1University Freiburg
Medical Center, Department of Exercise Medicine and
Sport, University of Freiburg, Freiburg, Germany
2Department of Dermatology, Universitair
Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB),
Brussels, Belgium
3Thermo Fisher Scientific, Phadia AB,
Uppsala, Sweden
4Department of Womens and Childrens Health,
Uppsala University, Uppsala, Sweden
5Thermo Fisher Scientific, Phadia GmbH,
Freiburg, Germany
6Department of Dermatology and Allergy,
Technische Universität München, Munich, Germany
7ZAUM-Center for Allergy and Environment,
Helmholtz Center Munich, Technische Universität München,
Munich, Germany
8Allergy Research Group, Department of
Dermatology, Medical Center, University of Freiburg,
Freiburg, Germany |
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Abstract |
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Background:
The ImmunoCAP ISAC
112 platform is the
only commercially
available molecular
allergy IgE
multiplex test. Data
on the comparison of
this rather novel
test with the
molecular singleplex
ImmunoCAP IgE
platform are
lacking.
Objective: To
compare the
multiplex ISAC 112
platform and the
singleplex ImmunoCAP
platform in regard
to IgE to grass
pollen allergens in
untreated grass
pollenallergic
patients in Germany.
Methods:
Serum samples from
101 adults with
grass pollen allergy
were analyzed for
specific IgE (sIgE)
to 8 allergenic
molecules from
timothy grass pollen
and to the 112
allergenic molecules
included in the ISAC
panel. The results
for the multiplex
and singleplex tests
were subsequently
analyzed
statistically.
Results:
Comparison of sIgE
to grass pollen
allergens detected
by ISAC 112 and the
singleplex ImmunoCAP
assay revealed the
following
correlation
coefficients: 0.88 (rPhl
p 1), 0.96 (rPhl p
2), 0.70 (nPhl p 4),
0.94 (rPhl p 5b),
0.92 (rPhl p 6),
0.85 (rPhl p 11),
and 0.78 (rPhl p12).
Conclusion:
Molecular testing
with ISAC 112
correlates well with
the ImmunoCAP
platform for
respective molecular
timothy grass pollen
allergens.
Key words:
Molecular allergy.
Multiplex. ImmunoCAP.
ISAC.
Component-resolved
diagnosis. |
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