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López-Matas MA1,
Larramendi CH2, Huertas AJ3,
Ferrer A4, Moya R1, Pagán JA5,
Navarro LA6, García-Abujeta JL2,
Carnés J1 |
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1R&D Department,
Laboratorios LETI S.L., Tres Cantos, Madrid, Spain
2Allergy Section, Hospital Marina Baixa,
Villajoyosa and Centro de Especialidades Foietes,
Benidorm, Alicante, Spain
3Allergy Section, Complejo Hospitalario
Universitario de Cartagena, Cartagena, Murcia, Spain
4Allergy Unit, Hospital de la Vega Baja,
Orihuela, Alicante, Spain
5Allergy Service, Hospital Virgen de la
Arrixaca, Murcia, Spain
6Allergy Unit, Centro de Especialidades El
Españoleto, Játiva, Spain |
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Abstract |
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Background:
Tomato allergies
have been
extensively studied
but
component-resolved
in vivo diagnosis
with purified
allergens has yet to
be performed.
Objectives:
To evaluate the
prevalence of
sensitization to
Sola l 3 in a
Mediterranean
population, and to
compare the
resulting
sensitization
profile with that of
individuals
sensitized to
tomato, peach,
and/or purified
lipid transfer
protein (LTP).
Methods: Sola
l 3 was purified,
characterized, and
used to prepare skin
prick tests (SPTs).
Two groups of
patients were
selected. Group 1
consisted of
patients with at
least 1 positive SPT
to tomato, peach, or
LTP mixture (marker
extracts) who were
subsequently tested
with Sola l 3
(n=280). Group 2
(prevalence study)
consisted of
patients who
underwent
simultaneous SPT
with the 3 marker
extracts and Sola l
3 (n=658). Patients
from either group
who were positive to
any of the 4
extracts were
studied in detail
(study group,
n=123). ELISA and
immunoblot assays
were performed in
individuals with a
positive SPT to Sola
l 3 to detect the
presence of specific
IgE antibodies to
this allergen.
Results:
Prevalence of
sensitization to
Sola l 3 was 3.2%
overall and 54.7% in
tomato-positive
patients. Most
tomato-sensitized
patients were
asymptomatic.
Symptoms were more
common in Sola l
3positive
individuals.
Sensitization to
peach and the LTP
mixture did not
discriminate between
Sola l 3positive
and Sola l
3negative patients.
Conclusions:
This study confirms
that LTP, not only
from peach but also
from other fruit and
vegetables,
including tomato, is
an important
allergen in the
Mediterranean area.
Sensitization to
Sola l 3 is
associated with more
symptoms in
tomato-sensitized
patients.
Key words:
Tomato. Peach. nsLTP.
Sola l 3. Allergens.
In vivo diagnosis.
Food allergy.
Molecular diagnosis. |
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