Background: Exposure to airborne allergens of biological origin is associated with the development and exacerbation of allergic asthma and rhinitis. Assessment of allergen exposure in health care facilities may improve monitoring of hygiene and surveillance of specific allergens that can cause symptoms in sensitized persons.
Objective: To assess concentrations of airborne food and aeroallergens in various health care settings in Portugal.
Methods: Dust was vacuumed from primary health care centers in Lisbon and from the emergency department, day hospital, internal medicine ward, operating room, and outpatient clinic of a university central hospital in Porto. Samples were sieved, weighed, and extracted, and concentrations of Nbos d 5, Cor a 9, Gal d 2, Ara h 3, Ara h 6, Der p 1, Fel d 1, Can f 1, Bla g 2, Alt a 1, and Phl p 5 were determined using a multiplex array for allergens (MARIA).
Results: All airborne food and aeroallergens were found in at least 1 sampled area, except for Alt a 1. Levels of Der p 1 and Fel d 1 ranged from 13.0 μg/g to 971.0 μg/g and from 7.0 μg/g to 4618.8 μg/g, respectively. Higher levels of food allergens were found in the emergency department (Nbos d 5, 16 034.0 μg/g; and Cor a 9, 10 649.5 μg/g).
Conclusions: Except for the operating room, exposure levels for dust mite and cat and dog dander in health care facilities were above the values associated with sensitization and allergic asthma or rhinitis symptoms in sensitized persons.

Key words: Airborne food allergens, Allergic disease, Health care centers, Aeroallergens, Indoor exposure